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pLDR21 [WM2194]
pLDR21 [WM2194]
規(guī)格:
貨期:
編號(hào):B217281
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pLDR21 [WM2194]
商品貨號(hào) B217281
Designations pLDR21 [WM2194]
Depositors W Messer
Biosafety Level 1
Vector Information
Size (kb): 3.6500000953674320
Vector: pLDR21 (plasmid)
Promoters: Promoter ptac
Construction: pT7-7, pJF118EH
Marker(s):ampR
Construct size (kb): 3.650000095367432
Features: E. coli promoter cassette: lacIq - ptac cassette
marker(s): ampR
operator: lac
promoter: ptac
replicon: pMB1
repressor gene: lacIq
Applications
contains easily purifiable cassette(s) for construction lacIq - ptac promoter cassette
Comments
Restriction digests of the clone give the following sizes (kb): BglI--4.0; ClaI--4.0; XhoI--2.3, 1.65.
Helper plasmid with an XhoI promoter cassette containing the gene for lacIq and the ptac promoter.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Diederich L, et al. A versatile plasmid vector system for the regulated expression of genes in Escherichia coli. BioTechniques 16: 916-920, 1994. PubMed: 8068348

Shipped freeze-dried
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