| 產(chǎn)品名稱 | HBE4-E6/E7 [NBE4-E6/E7] |
|---|---|
| 商品貨號(hào) | B217353 |
| Organism | Homo sapiens, human |
| Tissue | lung; bronchus |
| Cell Type | epithelial; human papillomavirus 16 (HPV-16) E6/E7 transformed |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 2 [Cells contain human papilloma viral DNA sequences]
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | normal |
| Age | 60 years |
| Gender | male |
| Ethnicity | Caucasian |
| Storage Conditions | liquid nitrogen vapor phase |
| Karyotype | 45, X, -Y, dup (5), -8, +9, -20, -22, +mar1, +mar2 |
| Derivation | The HBE4-E6/E7 (formerly NBE4-E6/E7) cell line was derived from normal bronchial epithelium taken from a man undergoing a left upper lobectomy for a poorly differentiated (T2 N0) adenocarcinoma. Cells from the primary explant in their first passage were transfected with the p1321 plasmid encoding the E6 and E7 genes of Human Papilloma virus type 16 (HPV 16) under the control of the human beta actin promotor. |
| Clinical Data | 60 years
Caucasian
male
|
| Tumorigenic | No |
| Effects | No, not tumorigenic in nude mice |
| Comments | These sequences are known to bind to and inactivate endogenous p53 and Rb proteins respectively.
Southern blot analysis shows that stable integration of the transfected genes occurred.
The cell line resembles morphologically the basal cells of the normal human bronchial epithelium, and is not tumorigenic in athymic nude mice.
The cells are able to form tubules when grown in a basement membrane like matrix, and they retain the ability to undergo terminal squamous differentiation in response to phorbol esters or upon reaching confluence.
Cells are non-viable in DMSO and should be frozen in culture Medium with 10% glycerol.
Original authentication testing at ATCC by isoenzymology indicated that this was a human cell line. Recent additional speciation using a mitochondrial cytochrome c oxidase I (CO1) assay has shown that this cell line is cross-contaminated with bovine cells. Additional more sensitive PCR assays of the original token lot have also indicated a low level of bovine cross-contamination in that material as well. |
| Complete Growth Medium | The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF).
To make the complete growth medium, you will need to add the following components to the base medium:
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture before the cells become confluent.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation | culture medium, 90%; glycerol, 10% |
| Culture Conditions | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| STR Profile | Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11 D16S539: 11,13 D5S818: 10,11 D7S820: 10,11 THO1: 6,8 TPOX: 8 vWA: 14,17 |
| Population Doubling Time | 24 hrs |
| Name of Depositor | J Viallet |
| Deposited As | Homo sapiens |
| Passage History | Cells from the primary explant in their first passage were transfected with the p1321 plasmid encoding the E6 and E7 genes of Human Papilloma virus type 16 (HPV 16) under the control of the human beta actin promotor. |
| References | Viallet J, et al. Characterization of human bronchial epithelial cells immortalized by the E6 and E7 genes of human papillomavirus type 16. Exp. Cell Res. 212: 36-41, 1994. PubMed: 8174640 Tsao MS, et al. Autocrine growth loop of the epidermal growth factor receptor in normal and immortalized human bronchial epithelial cells. Exp. Cell Res. 223: 268-273, 1996. PubMed: 8601403 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |
| 序號(hào) | 文章標(biāo)題 | 操作 |
|---|---|---|
| 1 | ATCC細(xì)胞運(yùn)輸和處理 | 查看詳情 |
| 2 | 其他海外品牌代理 | 查看詳情 |
| 3 | ATCC代理 | 查看詳情 |
| 4 | 購(gòu)買須知 | 查看詳情 |
| 5 | 訂購(gòu)流程 | 查看詳情 |
| 梅經(jīng)理 | 17280875617 | 1438578920 |
| 胡經(jīng)理 | 13345964880 | 2438244627 |
| 周經(jīng)理 | 17757487661 | 1296385441 |
| 于經(jīng)理 | 18067160830 | 2088210172 |
| 沈經(jīng)理 | 19548299266 | 2662369050 |
| 李經(jīng)理 | 13626845108 | 972239479 |
