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pAB366-2
pAB366-2
規(guī)格:
貨期:
編號(hào):B218526
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱(chēng) pAB366-2
商品貨號(hào) B218526
Designations pAB366-2
Depositors BM Tyler
Biosafety Level 1
Vector Information
Size (kb): 4.4000000953674320
Vector: pAB366-2 (plasmid)
Construction: pAB366 (ATCC 77134)
Marker(s):ampR bleR
Construct size (kb): 4.400000095367432
Features: marker(s): ampR bleR
replicon: pMB1
Applications
shuttle vector
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--2.9, 1.5; BamHI/XhoI--3.6, 0.8; BglI--3.0, 1.4.
Expression of ble (bleomycin resistance) is regulated by the promoter and terminator regions of the am gene (amination, glutamate dehydrogenase) from N. crassa.
Shuttle vector conferring bleomycin and phleomycin resistance.
Differs from pAB366 (ATCC 77134) by the insertion of a NsiI cloning site in the 5' untranslated region of ble to permit transcriptional fusion.
The order of the major features in this plasmid is: pTZ19R - EcoRI - BamHI - am promoter - NsiI - ble - XhoI - am 3' region - HindIII - pTZ19R.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Austin B, et al. Optimized vectors and selection for transformation of Neurospora crassa and Aspergillus nidulans to bleomycin and phleomycin resistance. Gene 93: 157-162, 1990. PubMed: 1699844

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