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pGB2862
pGB2862
規(guī)格:
貨期:
編號(hào):B218879
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGB2862
商品貨號(hào) B218879
Designations pGB2862
GenBank Number

M17697

Species Homo sapiens, human
Applications
This clone encodes conserved epitopes because it was isolated from an expression library by anti-bovine glutamate dehydrogenase antibodies.
Vector
Construct size (kb): 5.0
Insert
DNA: cDNA
Insert lengths(kb): 1.799999952316284
Tissue: brain stem
Gene product: glutamate dehydrogenase 1 [GLUD]
Insert Size (kb) 1.800
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
The insert contains the following sites separated by (bp)(approx): EcoRI- 340- BglII- 50- BglII- 210- EcoRV- 305- PstI- 920- EcoRI.
Restriction digests of the clone give the following sizes (kb): EcoRI--3.0, 2.1; BamHI--5.0; HincII--3.2, 1.4 + smaller; BglII--5.2; XbaI--5.2.
The insert contains a 774 bp open reading frame which codes for amino acids 244-501 of human liver GDH, and about 1 kb of untranslated sequence.
The cDNA sequence of human brainstem glutamate dehydrogenase is 95% homologous to the human liver sequence and 97% homologous to the bovine liver sequence.
This probe detects a single 3.7 kb band in human brain and glioma mRNA. In rat it detects a 3.5 kb band in liver, brain and C6 glioma cells, a 2.8 kb band in kidney, and a reduced 2.8 kb band in brain total mRNA.
This clone encodes conserved epitopes because it was isolated from an expression library by anti-bovine glutamate dehydrogenase antibodies.
References

Banner C, et al. Isolation of a human brain cDNA for glutamate dehydrogenase. J. Neurochem. 49: 246-252, 1987. PubMed: 3585334

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