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pG57/T1
pG57/T1
規(guī)格:
貨期:
編號:B219559
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pG57/T1
商品貨號 B219559
Designations pG57/T1
GenBank Number

M61773

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors A Tzagoloff
Applications
in another host, produces protein mitochondrial protein, required for assembly of F1 ATPase
Vector
Construct size (kb): 16.79998970031738
Insert
DNA: genomic
Insert lengths(kb): 9.0
Gene product: mitochondrial protein, required for assembly of F1 ATPase [ATP12]
Target Gene: mitochondrial protein, required for assembly of F1 ATPase
Insert Size (kb) 9.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Restriction digests of the clone give the following sizes (kb): SmaI/SalI--10.0, 8.0; EcoRI--6.6, 3.6 (doublet), 2.3, 0.9; PstI--13.5, 2.9.
The insert contains the following restriction sites (approximate kb from the end closer to the vector SmaI site [nt of the published sequence]): XbaI--1.1, 2.8; EcoRI--1.6, 5.3, 6.3 [193]; SstI--6.1 [2]; HindIII--7.5 [1425].
The EcoRI site at nt 193 is within the reading frame.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Bowman S, et al. Characterization of ATP12, a yeast nuclear gene required for the assembly of the mitochondrial F1-ATPase. J. Biol. Chem. 266: 7517-7523, 1991. PubMed: 1826907

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