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pbetaHalpha-5 [pBHA-5]
pbetaHalpha-5 [pBHA-5]
規(guī)格:
貨期:
編號(hào):B220870
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pbetaHalpha-5 [pBHA-5]
商品貨號(hào) B220870
Designations pbetaHalpha-5 [pBHA-5]
GenBank Number

M16411

Species Homo sapiens, human
Applications
Five fragments have been used as probes: 1.4 kb KpnI, 0.31 kb HincII, 0.257 kb SacI, 0.569 kb EcoRI/SacI, and 0.388 kb PvuII/SacI.
Vector
Construct size (kb): 6.0
Insert
DNA: cDNA
Insert lengths(kb): 1.944000005722046
Tissue: adult liver
Gene product: hexosaminidase A (alpha polypeptide)( Hexose aminidase A (alpha polypeptide), beta-N-acetylhexosaminidase, N-acetyl-beta-glucosaminidase) [HEXA]
Alleles: A2, A1, A1, A2
Insert Size (kb) 1.944
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Shipping Information Distributed: DNA distributed as 5 ug in 50 ul TE (volume: 50 ul,
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--6.0; HindIII--6.0; PstI--4.0, 2.0; AccI--4.8, 1.5; BamHI--6.0.
Insert includes 168 bp 5' untranslated sequence, 1587 bp of open reading frame, and 186 bp 3' untranslated, encoding a protein of 529 amino acids, 60.7 kDa.
There is a 5' deletion of 5-8 kb in a French-Canadian Tay-Sachs population.
Recorded as not polymorphic by Collaborative Research, meaning either not polymorphic in 0.8% agarose gels, or difficult to work with.
Five fragments have been used as probes: 1.4 kb KpnI, 0.31 kb HincII, 0.257 kb SacI, 0.569 kb EcoRI/SacI, and 0.388 kb PvuII/SacI.
References

Myerowitz R, Hogikyan ND. Different mutations in Ashkenazi Jewish and non-Jewish French Canadians with Tay-Sachs disease. Science 232: 1646-1648, 1986. PubMed: 3754980

Myerowitz R, et al. Human beta-hexosaminidase alpha chain: Coding sequence and homology with the beta chain. Proc. Natl. Acad. Sci. USA 82: 7830-7834, 1985. PubMed: 2933746

Nakai H, et al. Assignment of beta-hexosaminidase A alpha-subunit to human chromosomal region 15q24----q24. Cytogenet. Cell Genet. 56: 164, 1991. PubMed: 1829032

Myerowitz R. Splice junction mutation in some Ashkenazi Jews with Tay-Sachs disease: evidence against a single defect within this ethnic group. Proc. Natl. Acad. Sci. USA 85: 3955-3959, 1988. PubMed: 3375249

Barbara Weiffenbach, personal communication

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