| 產(chǎn)品名稱 |
Herpetomonas sp. |
| 商品貨號(hào) |
B221996 |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
human, female liver biopsy, Corpus Christi, TX, 1976 |
| Product Format |
frozen |
| Type Strain |
no |
| Comments |
Unique no-branched polyunsaturated fatty acids Cyclopropane fatty acid |
| Medium |
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 1011: Diphasic blood agar medium
ATCC® Medium 947: L diphasic blood agar (ATCC Medium 1011) with Locke's solution overlay reduced from 3.0 ml to 1.0 ml
|
| Growth Conditions |
Max Temperature: 37.0°C Min Temperature: 25.0°C Duration: axenic Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Subcultivation |
Protocol: ATCCNO: 11745 SPEC: See general instructions for thawing and storage of frozen material before proceeding. Add thawed contents to a single 16 x 125 mm glass screw-capped test tube of the appropriate medium. Incubate the culture vertically with the cap screwed on tightly. It is essential to establish cultures initially in small volumes. Once established, the culture can be scaled up to larger volumes. Vigorously agitate the culture and aseptically transfer 0.1 ml of culture to a fresh tube of medium weekly. |
| Name of Depositor |
RB McGhee |
| Chain of Custody |
ATCC <<--RB McGhee<<--CDC <<--- Spohn Hospital |
| Year of Origin |
1976 |
| References |
McGhee RB, Cosgrove WB. Biology and physiology of the lower Trypanosomatidae. Microbiol. Rev. 44: 140-173, 1980. PubMed: 6997722
Fish WR, et al. The cyclopropane fatty acid of trypanosomatids. Mol. Biochem. Parasitol. 3: 103-115, 1981. PubMed: 7254247
Fish WR, et al. Some Phytomonas and Herpetomonas species form unique iso-branched polyunsaturated fatty acids. Mol. Biochem. Parasitol. 5: 1-18, 1982. PubMed: 7062937
|
