Singhamoeba horticola (Singh and Hanumaiah) Sawyer et al.
商品貨號(hào)
B228737
Deposited As
Schizopyrenus horticolus Singh and Hanumaiah
Strain Designations
CR-1-3
Biosafety Level
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
bottom sediment, Chester River, MD, 1985
Product Format
dried
Type Strain
no
Comments
species description
Medium
ATCC® Medium 802: Sonneborn's Paramecium medium
Growth Conditions
Temperature: 25.0°C
Duration: grown with Escherichia coli ATCC 11775
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Subcultivation
Protocol: ATCCNO: 30133 SPEC: This strain is shipped as a dried preparation. Aseptically add 1 ml of sterile distilled water to the inner vial, remove the filter paper pellet with a pair of forceps, and place it in the center of an agar plate of ATCC medium 711 or 997. Add the liquid remaining in the vial to the plate and spread it evenly over the surface of the plate. Incubate the plate at 25C. Trophozoites (amebae) should be evident within 2-3 days.
Name of Depositor
TK Sawyer
Year of Origin
1985
References
Sawyer TK, et al. Singhamoeba horticala new combination Singh and Hanumaiah 1979 type species of Singhamoeba new genus. J. Protozool. 39: 107-109, 1992.
