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pGIKS-CRE
pGIKS-CRE
規(guī)格:
貨期:
編號:B229541
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 pGIKS-CRE
商品貨號 B229541
Designations pGIKS-CRE
GenBank Number

X03453

Species Staphylococcus aureus subsp.aureus bacteriophage P1
Depositors M Follettie
Applications
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
Vector
Construct size (kb): 3.9
Vector end (5'): KpnI
Vector end (3'): SacI
Excise insert: XhoI+SmaI
Promoter giving the sense strand: T3
Promoter giving the antisense strand: T7
Insert
DNA: genomic
Insert lengths(kb): 1.0
Gene product: recombinase, site-specific [cre]
Insert end (5'): KpnI linker
Insert end (3'): SacI
Insert Size (kb) 1.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Comments
For newer versions of the microarray control probes, please see a set of clones genes: ATCC 87840 (kit).
The insert contains nt 485-1516 of Genbank X03453.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.36; PvuI--0.46; ClaI--0.81.
The following restriction enzymes are recommend for linearizing the plasmid for in vitro transcription: T3 = SmaI; T7 = XhoI.
Restriction digests of the clone give the following sizes (kb): SmaI--4.0; SmaI/XhoI--3.0, 1.1; XhoI--4.0.
梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479